Sirtuin 6 protects human retinal pigment epithelium cells from LPS-induced inflammation and apoptosis partly by regulating autophagy |
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Authors: | Jingjing Liu Dan Liu |
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Affiliation: | 1. Department of Ophthalmology, Jinzhou He Eye Specialist Hospital , Jinzhou, P.R. China;2. Department of Ophthalmology, The First Affiliated Hospital of Jinzhou Medical University , Jinzhou, P.R. China liujjing2020@163.com;4. Department of Ophthalmology, The First Affiliated Hospital of Jinzhou Medical University , Jinzhou, P.R. China |
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Abstract: | ABSTRACT Lipopolysaccharides (LPS)-induced retinal inflammation is an important factor in retinal diseases. This study was aimed to investigate the effect of Sirt6 on LPS-induced retinal injury. ARPE-19 cells were incubated with LPS to induce inflammation. The cell viability was determined using CCK-8 assay. The mRNA level and protein expression of corresponding genes was detected using qRT-PCR and western blot, respectively. The production of inflammatory cytokines was measured using ELISA kit. The levels of oxidative stress-related factors were measured using their detection kits. Cell apoptosis was observed using TUNEL assay. The results showed that Sirt6 was downregulated after LPS treatment. Sirt6 strengthened LPS-induced autophagy by promoting the expression of LC3II/I, beclin1 and ATG5. Sirt6 treatment significantly inhibited LPS-induced inflammation, oxidative stress and cell apoptosis, which was then partly abolished by 3 MA. These results suggest Sirt6 to be an important regulator for LPS-induced inflammation, oxidative stress, and apoptosis partly by regulating cell autophagy. |
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Keywords: | Sirtuin 6 human retinal pigment epithelium cells inflammation apoptosis autophagy |
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