Development of a novel anti-biofilm peptide derived from profilin of Spodoptera frugiperda |
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| Authors: | Amanda Carolina Borges da Silva Janaina de Cassia Orlandi Sardi Daniella Gorete Lourenço de Oliveira Caio Fernando Ramalho de Oliveira Helder Freitas dos Santos Edson Lucas dos Santos |
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| Institution: | 1. Protein Purification Laboratory and Biological Functions, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil;2. Center for Biotechnology and Bioprospecting Studies Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil |
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| Abstract: | AbstractCandida yeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide—PEP-IA18—was evaluated against Candida species. PEP-IA18 was designed from the primary sequence of profilin, a protein from Spodoptera frugiperda, and displayed potent activity against Candida albicans and Candida tropicalis, showing a minimum inhibitory concentration (MIC) of 2.5?µM. Furthermore, the mechanism of action of PEP-IA18 involved interaction with the cell membrane (ergosterol complexation). Treatment at MIC and/or 10?×?MIC significantly reduced biofilm formation and viability. PEP-IA18 showed low toxicity toward human fibroblasts and only revealed hemolytic activity at high concentrations. Thus, PEP-IA18 exhibited antifungal and anti-biofilm properties with potential applicability in the treatment of infections caused by Candida species. |
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| Keywords: | Antifungal biofilm Candida spp peptide |
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