The antitumor molecular mechanism of Alisma orientalis with c-myc DNA: multi-spectroscopic analysis and molecular simulation |
| |
Authors: | Fei Xu Jun Chen Qinan Wu Yuqing Shen Cai Lu |
| |
Affiliation: | 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China;2. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China;3. Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, China;4. Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, China;5. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing, China;6. Department of Neurology, Taizhou Second People’s Hospital, Taizhou, China |
| |
Abstract: | Abstract We prepared extracts of Alisma orientalis from Sichuan and Fujian Province, China. Based on the ratio of alisol B 23-acetate (23B) to alisol A 24-acetate (24A) in two Alisma orientalis extracts, we prepared two mixtures of 24A and 23B (24A:23B?=?1:3 or 1:10). The antitumor molecular mechanism of the monomers 24A and 23B, the two mixtures and the effective components of Alisma orientalis from different habitats were studied. The MTT assay suggested that the difference in the antitumor activity of Alisma orientalis from different habitats was correlated to the ratio of 24A to 23B. The multi-spectroscopic analysis suggested that the effective components, the monomers and mixtures interacted with c-myc DNA in a partial intercalation manner. The binding strength of the alisol acetates to c-myc DNA was consistent with the anticancer activity, indicating that c-myc DNA was the anticancer target. The molecular simulation indicated that the mixtures were all directly bound to different base pairs of c-myc DNA for a superimposed effect, which led to the binding strength of the mixtures to c-myc DNA was stronger than that of the monomers. The molecules in the 1:3 mixture were all bound to different base pairs of c-myc DNA. However, for the 1:10 mixture, seven molecules of 23B bound to the side chain of 24A, resulting in the mixture with a long chain structure which increased the steric hindrance of 24A. As a result, affinity between 24A and c-myc DNA in the 1:10 mixture was weaker than that in the 1:3 mixture. | |
Keywords: | Alisma orientalis antitumor molecular mechanism c-myc DNA multi-spectroscopic analysis molecular simulation |
|
|