Diversity of amoA and pmoA Genes in Extremely Saline Alkaline Soils of the Former Lake Texcoco |
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| Authors: | Valentín Pérez-Hernández Mario Hernández-Guzmán Nancy Serrano-Silva Marco Luna-Guido Yendi Ebenezer Navarro-Noya Joaquín Adolfo Montes-Molina |
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| Affiliation: | 1. Laboratory of Soil Ecology, Department of Chemistry and Biochemistry, Instituto Tecnológico de Tuxtla-Gutiérrez, Tecnológico Nacional de México, Tuxtla-Gutiérrez, Mexico;2. Laboratory of Soil Ecology, Cinvestav, Mexico City, Mexico;3. Departamento de Biología de la Conducta, CONACYT-Tlaxcala Autonomous University, Tlaxcala, Mexico |
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| Abstract: | Abstract Ammonia-oxidizing bacteria (AOB) and aerobic methane oxidizing-bacteria (MOB) were studied in three extreme soils of the former Lake Texcoco, Mexico, with pH ranging from 8.5 to 10.5 and electrolytic conductivity (EC) from 0.67 to 84.76 dS m?1, and in two arable soils. Soil DNA was extracted with three different methods and total DNA was used as a template to amplify the pmoA and amoA functional genes and subsequently sequenced by pyrosequencing. The amoA gene sequences clustered as uncultured AOB dominated in the Texcoco soils, while Nitrosospira was dominant in the arable soils. Sequences of MOB associated with Nitrosococcus-rel (Type I) dominated (>85%) in the Texcoco soils, but they were more diverse in the arable soils, for example, JR2, JR3, Methylocaldum USC-g (Type I), USC-a (Type II) and gp23 (pxmA). Aerobic methane oxidizing-bacteria and AOB microbial diversity were significantly related to EC and pH (p?
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| Keywords: | Ammonia oxidizing bacteria extreme environment methane-oxidizing bacteria pyrosequencing saline-alkaline soils |
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