Antioxidant activity and absorption of cyanidin-3-O-glucoside liposomes in GES-1 cells in vitro |
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| Authors: | Zhao Quan Haizhi Huang Kai Yang Ming Cai Xianghe Meng |
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| Institution: | 1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou, China https://orcid.org/0000-0002-1002-3789;2. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou, China;3. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, P. R. Chinahttps://orcid.org/0000-0001-5571-6645;4. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, P. R. Chinahttps://orcid.org/0000-0003-0782-9877;5. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, P. R. Chinahttps://orcid.org/0000-0001-6934-3675 |
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| Abstract: | ABSTRACTThe use of anthocyanins are limited by their chemical properties. Recent evidence suggests Cyanidin-3-O-glucoside (C3 G) liposomes via the ethanol injection method exhibit improved stability. In the current study, the characterization and cell absorption of C3 G liposomes were explored via transmission electron microscopy and flow cytometry. The internalization of the C3 G liposomes across the gastric epithelial cell monolayer (GES-1 cells) were investigated. Results showed that the particle size and encapsulation efficiency were 234 ± 9.35 nm and 75.0% ± 0.001, respectively. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were used to evaluate the antioxidant activity of C3 G liposomes. The C3 G liposomes can obviously increased T-AOC and decreased the MDA content.Collectively, C3 G liposomes protected human GES-1 cells from gastric mucosal injury induced by H2O2 by activating the related antioxidant pathway. Our research could provide a new effective treatment strategy for the absorption of stomach drugs.Abbreviations: C3G: Cyanidin-3-O-glucoside; LP: Liposome; GES-1 cells: Human gastric epithelial cell lines; FBS: Fetal Bovine Serum; PBS: Phosphate-buffered saline; PC: Phosphatidylcholine; CH: Cholesterol; MDA: Malondialdehyde; TEM: Transmission electron microscope; FCM: Flow cytometry; FITC: Fluorescein isothiocyanate; DAPI: 4′, 6-diamidino-2phenylidole; FT-IR: Fourier Transform infrared spectroscopy; PFA: Paraformaldehyde |
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| Keywords: | Cyanidin-3-O-glucoside ges-1 cells liposome antioxidant absorption |
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![ORCID Icon]({"type":"image","src":"/templates/jsp/images/orcid.png"} "ORCID Icon")
https://orcid.org/0000-0002-1002-3789;2. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou, China;3. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, P. R. China