The effect of gold and silver nanoparticles,chitosan and their combinations on bacterial biofilms of food-borne pathogens |
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| Authors: | Ondrej Chlumsky Sabina Purkrtova Hana Michova Viviana Svarcova Petr Slepicka Dominik Fajstavr |
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| Institution: | 1. Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Czech Republic;2. chlumsko@vscht.cz;4. Department of Solid State Engineering, University of Chemistry and Technology, Prague, Czech Republic |
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| Abstract: | AbstractThe antimicrobial activity of gold and silver nanoparticles (AuNPs, AgNPs), chitosan (CS) and their combinations was established by determining the minimum inhibitory concentration for planktonic (MICPC80) and biofilm growth (MICBC80), for biofilm formation (MICBF80), metabolic activity (MICBM80) and reduction (MICBR80), and for the metabolic activity of preformed biofilm (MICMPB80). Biofilms were quantified in microtitre plates by crystal violet staining and metabolic activity was evaluated by the MTT assay. Chitosan effectively suppressed biofilm formation (0.31–5?mg ml?1) in all the tested strains, except Salmonella enterica Infantis (0.16–2.5?mg ml?1) where CS and its combination with AgNPs induced biofilm formation. Nanoparticles inhibited biofilm growth only when the highest concentrations were used. Even though AuNPs, AgNPs and CS were not able to remove biofilm mass, they reduced its metabolic activity by at least 80%. The combinations of nanoparticles with CS did not show any significant positive synergistic effect on the tested target properties. |
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| Keywords: | gold nanoparticles silver nanoparticles chitosan biofilm antimicrobial activity |
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