MiR-375-3p alleviates the severity of inflammation through targeting YAP1/LEKTI pathway in HaCaT cells |
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| Authors: | Shaohang Cheng Zhenghong Di Abdul Razaq Hirman Heng Zheng Linna Duo Qianyu Zhai |
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| Institution: | 1. Department of Dermatology, Shengjing Hospital of China Medical University , Shenyang, People’s Republic of China;2. Department of Dermatology, The Central Hospital Affiliated to Shenyang Medical College , Shenyang, People’s Republic of China |
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| Abstract: | ABSTRACT Atopic dermatitis (AD) is a relapsing inflammatory skin disease with a complicated pathogenesis. This study aimed to investigate whether miR-375-3p could regulate AD through the Yes-associated protein 1 (YAP1) pathway. In this study, inflammatory response was induced by TNF-α and IFN-γ administration in HaCaT cells. We found that viability and inflammatory factor release, including interleukin-1β (IL-1β) and IL-6, were negatively related to miR-375-3p expression in HaCaT cells. We also found that YAP1 overexpression down-regulated lympho-epithelial Kazal type inhibitor (LEKTI) levels and aggravated viability and inflammation in TNF-α and IFN-γ-treated HaCaT cells. Dual-luciferase reporter assay proved the targeted binding of miR-375-3p and YAP1 3?-UTR. Additionally, the protective effect of miR-375-3p on inflammatory response in TNF-α and IFN-γ-treated HaCaT cells could be impeded by YAP1 overexpression. Collectively, our results suggested that miR-375-3p could modulate HaCaT cell viability and inflammation through the YAP1/LEKTI pathway. |
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| Keywords: | Atopic dermatitis inflammation miR-375-3p YAP1 LEKT |
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