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| 农杆菌介导Bt基因遗传转化高粱 | |
| 引用本文： | 张明洲,唐乔,陈宗伦,刘军,崔海瑞,舒庆尧,夏英武,I. Altosaar. 农杆菌介导Bt基因遗传转化高粱[J]. 生物工程学报, 2009, 25(3): 418-423 |
| 作者姓名： | 张明洲 唐乔 陈宗伦 刘军 崔海瑞 舒庆尧 夏英武 I. Altosaar |
| 作者单位： | 1. 中国计量学院生命科学学院,杭州,310018 2. 浙江大学原子核农业科学研究所,杭州,310029 3. 渥太华大学生物化学系,加拿大 |
| 基金项目： | 国家自然科学基金项目(No.30270273);;浙江省自然科学基金项目(No.Y304463)资助~~ |
| 摘 要： | 高粱是全球仅次于小麦、水稻、玉米、大豆和马铃薯等的重要作物之一。以高粱幼穗愈伤组织为转化受体,通过农杆菌介导法和含有抗潮霉素和gus基因的双元载体将杀虫晶体蛋白基因cry1Ab导入高粱品种115、ICS21B和5-27,经Hyg筛选共获得21个独立的转基因株系,52株转基因植株,平均转化率为1.9%。经PCR、Southern杂交和RT-PCR分析表明cry1Ab基因已整合入高粱基因组中并得到正确转录。Bt蛋白Westernblotting分析和ELISA定量测定显示,cry1Ab基因在转基因高粱植株中表达,但不同转基因植株表达量有差异。饲虫试验表明,转基因高粱对大螟(Sesamiainferens)具有一定抗性。 |
| 关 键 词： | Bt基因 农杆菌 遗传转化 高粱 |
| 收稿时间： | 2008-10-17 |
Genetic transformation of Bt gene into sorghum (Sorghum bicolor L.) mediated by Agrobacterium tumefaciens |
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| Mingzhou Zhang,Qiao Tang,Zonglun Chen,Jun Liu,Hairui Cui,Qingyao Shu,Yingwu Xia and I. Altosaar. Genetic transformation of Bt gene into sorghum (Sorghum bicolor L.) mediated by Agrobacterium tumefaciens[J]. Chinese journal of biotechnology, 2009, 25(3): 418-423 | |
| Authors: | Mingzhou Zhang Qiao Tang Zonglun Chen Jun Liu Hairui Cui Qingyao Shu Yingwu Xia I. Altosaar |
| Affiliation: | College of Life Science, China Jiliang University, Hangzhou 310018, China;College of Life Science, China Jiliang University, Hangzhou 310018, China;College of Life Science, China Jiliang University, Hangzhou 310018, China;College of Life Science, China Jiliang University, Hangzhou 310018, China;Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China;Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China;Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China;Department of Biochemistry, University of Ottawa, Canada |
| Abstract: | Sorghum (Sorghum bicolor L.) was one of the most important crops in the world next to wheat, rice, maize, soybean and barley. Using the callus derived from immature inflorescence as the recipients, we efficiently transformed sorghum varieties 115, ICS21B and 5-27 with the insecticidal Bacillus thuringiensis (Bt) cry1Ab gene carried in the T-DNA of binary vectors which contained hygromycin resistance gene and gus gene via Agrobacterium tumefaciens. After gradient selection with hygromycin, a total of 21 inde... |
| Keywords: | Bt gene Agrobacterium tumefaciens genetic transformatian sorghum |
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