流感病毒双向扩散试验及其应用

用新甲1型流感病毒津防77-78兔免疫血清与SSS裂解的相应抗原进行双向扩散试验(简称双扩),出现两条明显的沉淀线,即血凝素和核蛋白沉淀线;而乙型Lee株抗原则不产生沉淀线。用双扩对新甲1型津防77-78与旧甲1型国内外代表株FM1、FW、京生53-7、京科55-3做单面的血凝素抗原分析,证明津防77-78的血凝素与FW一致,与其它毒株均有差异。但津防77-78的血凝素和核蛋白沉淀线的位置与FW的相反。 用酸沉淀处理流感病毒的方法浓缩抗原,与同型流感病毒免疫血清做双扩,可得到核蛋白沉淀线。用双扩和酸沉淀抗原能对新分离流感病毒满意地定型,方法简单,出结果快,优于补体结合试验。

IMMUNO-DOUBLE-DIFFUSION TEST OF INFLUENZA VIRUS AND ITS APPLICATION

An immuno-double-diffusion test was established using antisera from rabbitsimmunized with purified virus and sodium sarkosy] sulfate disrupted virus as antigen. Two well separated precipitin lines representing the hemagglutinin and the nucleoprotein were detected.Antigenic analysis of old and new H1N1 viruses by immuno-double-diffusion test using antiserum against one new HlNl strain ( Al/Tianjin/78/77 ) revealed that the hemagglutinin of Al/Tianjin/78/77 is different from that of A1/FM/1 /47, Al/Beijing/7/53,Al/Beijing/3/55, but closely similar to that of A1/FW/1 /50.However, the position of the hemagglutinin and the nucleoprotein precipitin lines of A1/FW/1/50 is reversed as compared with that of A1/Tianjin/78 /77.The reason of this was disscussed.A Simplified method of acid preparation of virus from the allantoic fluid yielded antigen preparations which gave only the nucleoprotein line. These antigens had been used successfully for typing new isolates of influenza virus.