Lipase-catalyzed transesterification in several reaction systems: An application of room temperature lonic liquids for bi-phasic production ofn-butyl acetate |
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Authors: | Suk-Chan Park Woo-Jin Chang Sang-Mok Lee Young-Jun Kim Yoon-Mo Koo |
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Institution: | (1) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea;(2) ERC for Advanced Bioseparation Technology, Inha University, 402-751 Incheon, Korea |
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Abstract: | Organic solvents are widely used in biotransformation systems. There are many efforts to reduce the consumption of organic
solvents because of their toxicity to the environment and human health. In recent years, several groups have started to explore
novel organic solvents called room temperature ionic liquids in order to substitute conventional organic solvents. In this
work, lipase-catalyzed transesterification in several uni-and bi-phasic systems was studied. Two representative hydrophobic
ionic liquids based on 1-butyl-3-methylimidazolum coupled with hexafluorophosphate (BMIM]PF6]) and bis(trifluoromethylsulfonyl) imide] (BMIM] Tf2N]) were employed as reaction media for the transesterification ofn-butanol. The commercial lipase, Novozym 435, was used for the transesterification reaction with vinyl acetate as an acyl
donor, The conversion yield was increased around 10% in a water/BMIM]Tf2N], bi-phasic system compared with that in a water/hexane system. A higher distribution of substrates into the water phase
is believed to enhance the conversion yield in a water/BMIM]Tf2N] system. Partion coefficients of the substrates in the water/BMIM]Tf2N] bi-phasic system were higher than three times that found in the water/hexane system, while n-butyl acetate showed a similar
distribution in both systems. Thus, RTILs appear to be a promising substitute of organic solvents in some biotransformation
systems. |
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Keywords: | ionic liquid lipase transesterification bi-phasic system butyl acetate |
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