Normal and malignant cells, including neurons, deposit plasminogen activator on the growth substrata |
| |
| Authors: | A Krystosek N W Seeds |
| |
| Institution: | 1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA;2. Department of Urology, University of Washington, Seattle, Washington, USA;3. Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA;4. Department of Radiology, University of Michigan, Ann Arbor, Michigan, USA;5. Division of Pediatric Cardiology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA;1. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Beijing Normal University, Xinjiekouwai Street No. 19, Beijing 100875, China;2. Department of Geographical Sciences, University of Maryland, 2181 LeFrak Hall, College Park, MD 20740, USA;3. Division of Environment and Sustainability, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China;1. Metabolic Disease Biology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India;2. Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India;3. Tissue Engineering and Regenerative Medicine Laboratory, Center for Biomedical Engineering, Indian Institute of Technology Ropar, Rupnagar, 140001, Punjab, India;4. Dibrugarh University, National Highway 37, Dibrugarh, 786004, Assam, India |
| |
| Abstract: | The results of four different assay methods showed that both normal and malignant plasminogen activator-secreting cells deposited substantial amounts of this protease on tissue-culture substrata, including collagen coatings. The cells studied were Rous sarcoma virus (RSV)-transformed vole fibroblasts, a malignant neural cell line (NG108-15) capable of neurite formation, and normal mouse-regenerating sensory neurons. Deposited plasminogen activator was detected by a fibrin overlay assay at sites from which cells growing on coverslips had been gently dislodged, showing that active enzyme is left beneath cells and in the immediate pericellular area. For neuronal cells, fibrinolytic zones were detected not only at the previous positions of cell bodies but also along the terrain conditioned by neurite extension, suggesting that a trail of plasminogen activator is left behind during growth cone movement. Substratum-bound enzyme could be solubilized in buffers containing sodium dodecyl sulfate (SDS) or Triton X-100 and demonstrated by zymography following electrophoresis or assayed for amidolytic activity with a chromogenic substrate (Kabi S-2251). The results suggest that plasminogen activator may be considered a component of substrate-adhesion material. Secretory proteases deposited directly on matrix molecules would seem strategically positioned to participate in local degradation of components of the extracellular environment. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
