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| RAPD标记在山葡萄种质鉴定中的应用 | |
| 引用本文： | 王军,葛玉香,贺普超.RAPD标记在山葡萄种质鉴定中的应用[J].植物研究,2004,24(4):473-476. |
| 作者姓名： | 王军 葛玉香 贺普超 |
| 作者单位： | 1. 东北林业大学, 哈尔滨 150040; 2. 西北农林科技大学, 杨凌 712100 |
| 基金项目： | 东北林业大学校科研和教改项目 |
| 摘 要： | 采用修改后的CTAB 法获得了高质量的基因组DNA 。利用RAPD 标记技术对山葡萄7 份种质进行鉴定, 用4 个引物(从30 个引物中筛选)对试材进行PCR 扩增, 共扩增出30 条谱带, 平均每条引物产生7.5 条谱带, 其中21 条谱带为多态性谱带, 占总谱带数的70%。不同引物扩增的谱带数不同, 范围在6~9 条之间。利用4 个引物扩增出的多态性谱带可以将7 份山葡萄种质区分。 |
| 关 键 词： | 山葡萄 种质 鉴定 RAPD |
| 收稿时间： | 2004-02-20 |
| 修稿时间： | 2004年2月20日 |
Application of RAPD markers to identification of Vitis amurensis germplasm |
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| WANG Jun,GE Yu-Xiang,HE,Pu-Chao.Application of RAPD markers to identification of Vitis amurensis germplasm[J].Bulletin of Botanical Research,2004,24(4):473-476. | |
| Authors: | WANG Jun GE Yu-Xiang HE Pu-Chao |
| Institution: | 1. Northeast Forestry University, Harbin 150040; 2. Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100 |
| Abstract: | High quality genomic DNA was obtained by the method of modified CTAB. Seven germplasms Vitis amurensis were identified by RAPD, four primers screened from 30 primers could amplify clearly and stably 30 bands, average was 7.5 bands. There in 21 of 30 bands were examined for random amplified polymorphic DNA amplification patterns. They occupied 70%. Every primer could amplify different bands, the range of the number of bands was from 6 to 9. The result indicated that polymorphic band amplified by the 4 primers was effective in identification 7 germplasms of Vitis amurensis. |
| Keywords: | RAPD |
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