The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application |
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| Authors: | Quanfeng Liang Qian Wang Cuijuan Gao Zhankun Wang Qingsheng Qi |
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| Institution: | (1) State Key Laboratory of Microbial Technology, Shandong University, 250100 Jinan, People’s Republic of China;(2) National Glycoengineering Research Center, Shandong University, 250100 Jinan, People’s Republic of China; |
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| Abstract: | Cyclodextrins (CDs) are used in food, pharmaceutical, and chemical industries, as well as agriculture and environmental engineering.
Cyclodextrin glucanotransferase (CGTase) is an important industrial extracellular enzyme which is used to produce CDs and
oligosaccharides. We previously developed a novel yeast-surface CGTase expression system which was used for the production
of CDs from starch. In the present study, we showed that the presence of CDs may increase the ethanol tolerance of microorganisms.
The cell numbers of Saccharomyces cerevisiae and Escherichia coli in the presence of β-cyclodextrin and ethanol were 1,000-fold and 10-fold higher than that without CDs. The yeast strain
with the immobilized CGTase produced 13 g CDs/l and 1.8 g ethanol/l when it was incubated in yeast medium supplemented with
4% starch. The effect of CDs on microorganisms suggests a potential application for the co-production of CDs and ethanol. |
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