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Optimization of nutrient medium for submerged cultivation of Ganoderma lucidum (Curt.: Fr.) P. Karst |
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| Authors: | A V Avtonomova L M Krasnopol’skaya V N Maksimov |
| Institution: | (1) Gauze Institute of New Antibiotics, Russian Academy of Medical Sciences, ul. Bol’shaya Pirogovskaya 11, Moscow, 119867, Russia;(2) Moscow State University, Vorob’evy gory, Moscow, 119992, Russia |
| Abstract: | The dependence of the amount of the grown vegetative mycelium of Ganoderma lucidum on the composition of the nutrient medium has been studied under conditions of submerged cultivation. The medium was optimized using full factorial and steepest ascent experimental designs. The addition of two carbon sources to the medium considerably improved the submerged growth of the mushroom. An optimized medium provided for a high yield (20-20.95 g/l) of the morphologically homogeneous mycelium and shortened the cultivation period to 3–4 days. |
| Keywords: | optimization submerged cultivation biomass Ganoderma lucidum |
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