Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12 |
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Authors: | Renato Morona Melissa H Brown Jane Yeadon Michael W Heuzenroeder Paul A Manning |
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Institution: | Enterovax Limited, Adelaide, South Australia. |
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Abstract: | The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region. |
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Keywords: | rfb Gene Transformation rfa Gene Glucose Silver stain Western blot Vibrio cholerae |
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