Analysis of data captured by an on-line image capture system from an analytical ultracentrifuge using schlieren optics

The recent development in this laboratory of an automated data capture system, for refractometric optics on the analytical ultracentrifuge has removed the requirement for tedious and time consuming manual acquisition which had led to a decline in the use of schlieren optics. At the same time this system has increased the amount of data easily available from such an optical system with maintained or increased precision. From the advent of such a system has arisen the need for a package to facilitate the analysis of these data and to extend the range of analytical methods used. Using the improved data sets now available has also enabled us to successfully use methods which have lapsed in popularity over the last two decades. We have also been able to successfully apply radial derivative methods (Bridgman 1942) which have not routinely been applied to the analysis of sedimentation velocity experiments using schlieren optics. In this paper we describe the methods we have so far used to analyse data and present results for previously well defined molecules to demonstrate that the results obtained are reliable. Received: 1 November 1996 / Accepted: 15 January 1997