Interaction of deoxyribonucleic acid with anthracenedione derivatives |
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Authors: | Manlio Palumbo Sebastiano Marciani Magno |
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Affiliation: | Biopolymer Research Centre, CNR, Institute of Organic Chemistry, and Centro di Studio sall Chimica del Farmaco e dei Prodotti Biologicamente Attivi, CNR, Institute of Pharmaceutical Chemistry, University of Padova, 35100 Padova, Italy |
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Abstract: | Spectrophotometric, calorimetric and chrioptical techniques have been used to investigate the interaction of two new anthracenedione derivatives, 1-(ω-diethylaminopropylamido)-4-hydroxy-9,10-anthracenedione hydrochloride (I) and 1-(ω)-diethylaminopropylamido)-2-methoxy-4-hydroxy-9,10-anthracenedione hydrochloride (II) to DNA. Measurements were carried out at four different Na+ concenetrations. From the dependence of the binding constant on ionic strength the number of ion pairs formed between the ligand and DNA, along with the binding free energy were estimated. Calorimetric measurements show that the binding process is exothermic for both ligands. Experiments carried out with DNA from various sources indicate no marked preference for G-C or A-T binding sites. Compounds I and II increase the Tm for DNA melting by more than 25°C at high drug/base pair ratios. Circular dichroism studies indicate that the structural properties of DNA are substantially affected by the interaction with the above mentioned compounds. All data from these studies are consistent with an intercalative mechanism of binding for the anthracendione derivatives to DNA. |
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Keywords: | DNA drugs anthracenedione intercalation circular dichroism |
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