Comparative analysis of whole genome structure of <Emphasis Type="Italic">Streptococcus suis</Emphasis> using whole genome PCR scanning |
| |
Authors: | Xiong ZhaoHui Wei CanDong Yang Jian Peng JunPing Xu XingYe Wang Yu Jin Qi |
| |
Institution: | 1.State Key Lab for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China;2.Chinese Center for Disease Control and Prevention,Beijing,China;3.Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing,China |
| |
Abstract: | An outbreak associated with Streptococcus suis infection in humans emerged in Sichuan province, China in 2005. The outbreak is atypical for the apparent large number of human cases, high fatality rate and geographical spread. To determine whether the bacterium has changed, we compared both human and animal isolates from the Sichuan outbreak with those collected previously within China and in other countries using whole genome PCR scanning (WGPScaning) comparative sequencing of several known virulence factor genes and multilocus sequence typing (MLST) analysis. WGPScanning analysis showed that all primer pairs yielded PCR products of the expected sizes in all four strains tested. The nucleotide sequences of all the detected virulence factor genes are identical in the four strains and MLST results showed that the four isolates studied and reference strain all belonged to the ST1 complex. No new genetic changes were found in the genome structure of the isolates from this Sichuan outbreak. |
| |
Keywords: | Streptococcus suis whole genome PCR scanning multilocus sequence typing (MLST) |
本文献已被 万方数据 SpringerLink 等数据库收录! |
|