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牛蒡提取液对副溶血性弧菌的抑制作用
引用本文:贺菊萍,赵勇,孙晓红,吴启华,潘迎捷. 牛蒡提取液对副溶血性弧菌的抑制作用[J]. 天然产物研究与开发, 2012, 24(3): 381-384
作者姓名:贺菊萍  赵勇  孙晓红  吴启华  潘迎捷
作者单位:1. 上海海洋大学食品学院,上海201306;徐州工程学院食品工程学院,徐州221008
2. 上海海洋大学食品学院,上海,201306
3. 上海海洋大学食品学院,上海201306;缅因大学食品科学与人类营养系,美国缅因,04469-5735
摘    要:研究了牛蒡提取物对副溶血性弧菌的抑制作用。采用80%乙醇分别浸提两个牛蒡品种柳川理想及黄肌牛蒡根的皮及去皮根,四种提取物分别命名为LP、LR、HP和HR。采用滤纸片扩散和最低抑菌浓度法筛选了四种提取物的抑菌效果,时变抑菌研究了常温(25℃)和低温(4℃)下牛蒡提取物在无菌蒸馏水(DW)以及脑心浸汤(BHI)中对副溶血性弧菌的抑制效果。结果显示,四种提取物中LP的抑菌活性最好;经1000μL/mL LP提取液7小时处理,在4℃和25℃DW环境均未有副溶血性弧菌检出;经各浓度提取液1天处理,在4℃和25℃BHI环境均未有副溶血性弧菌检出。LP对副溶血性弧菌弧菌具有良好的抑制作用。

关 键 词:牛蒡提取液  抑菌活性  副溶血性弧菌  时变抑菌

Antibacterial Effects of Burdock(Arctium Lappa L.) Concentrate on Vibrio parahemolyticus
HE Ju-ping , ZHAO Yong , SUN Xiao-hong , WU Qi-hua , PAN Ying-jie. Antibacterial Effects of Burdock(Arctium Lappa L.) Concentrate on Vibrio parahemolyticus[J]. Natural Product Research and Development, 2012, 24(3): 381-384
Authors:HE Ju-ping    ZHAO Yong    SUN Xiao-hong    WU Qi-hua    PAN Ying-jie
Affiliation:1* 1College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China; 2Food Department of Xuzhou Institute of Technology,Xuzhou 221008,China; 3Department of Food science and Human Nutrition,University of Maine,ME 04469-5735,USA
Abstract:The antibacterial activities of burdock roots against Vibrio parahemolyticus were investigated.The peel and peeled root of two varieties of burdock named Liuchuan and Huangji were extracted with 80% ethanol,classed as LP,LR,HP and HR respectively.The data from disk diffusion method and minimum inhibitory concentration(MIC) values showed that the antibacterial activity of LP was more significant than that of LR,HP and HR.The time–kill study indicated that V.parahemolyticus was reduced to non-detectable levels with 1000 μL/mL LP extract treatment at 4 and 25 ℃ for 7 h in DW and no bacteria cell was detected with all treatment on Day 1 at 4 and 25 ℃ in BHI.Basing on its good antibacterial activitiy,LP could be useful in control of V.parahemolyticus.
Keywords:Burdok concentrate  antibacterial effects  Vibrio parahemolyticus  time-dependent antibacterial
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