虎杖提取物对弹性蛋白酶的抑制作用研究

本文主要研究了虎杖提取物对弹性蛋白酶的抑制作用。实验利用热水浸渍法提取虎杖得到粗提取物1(CE1),聚酰胺柱层析后得到粗提取物2(CE2),并进行一系列的定性及定量分析。分别用紫外分光光度法和荧光光谱法研究了提取物对弹性蛋白酶的抑制作用和荧光猝灭作用。实验结果表明CE1和CE2中均含有虎杖苷,含量分别为41.01%和69.57%。CE1、CE2和虎杖苷对弹性蛋白酶有一定的抑制作用,当浓度为2.0 mg/mL时,它们对弹性蛋白酶的抑制率分别为53.56%、61.27%和82.53%。CE1、CE2和虎杖苷对弹性蛋白酶均有明显的内源荧光猝灭作用,当浓度为0.1 mg/mL时,荧光猝灭率分别为70.38%、72.90%和75.99%。

Elastase Inhibitory Activity of Extracts from Polygonum cuspidatum

The elastase inhibitory activity of extracts from Polygonum cuspidatum was studied in this paper.The crude extract 1(CE1) were extracted with hot water from P.cuspidatum.The crude extract 2(CE2) was separated from CE1 by using polyamide column chromatography.Extracts were identified by qualitative and quantitative analysis.The elastase inhibitory activity of extracts was studied by ultraviolet spectrum,and fluorescent spectrometry was used to investigate the interaction of elastase with the CE1,CE2 and polydatin.The results indicated the polydatin was the main component in CE1 and CE2,the content of polydatin were 41.01% and 69.59%.CE1,CE2 and polydatin all had significant inhibitory effect on elastase.When the concentration was 2.0 mg/mL,the inhibiting ratio of CE1,CE2 and polydatin were 53.56%,61.27% and 82.53%,respectively.Besides,these compounds had apparent endogenous fluorescence quenching effects.When the concentration was 0.1 mg/mL,the fluorescence quenching ratio of CE1,CE2 and polydatin were 70.38%,72.90% and 75.99%,respectively.