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Differential scanning calorimetry study on the inner membrane lipids prepared from barotolerant Pseudomonas sp. BT1
Authors:Kaneko H  Obuchi K  Horikoshi K
Institution:The DEEP STAR Group, Japan Marine Science and Technology Center, Natsushima-cho, Yokosuka 237-0061, Japan. kanekoh@jamstec.go.jp
Abstract:We investigated the properties of membrane lipids of barotolerant Pseudomonas sp. BT1 by differential scanning calorimetry and spectrophotometry using a system equipped with a hydrostatic pressure controller. In the case of cells grown under high pressure, an endothermic peak appeared under high-pressure measurement conditions. However, in the case of cells grown at 0.1 MPa, such a peak was not observed. It was also observed on spectrophotometry that the membrane lipids from cells grown at 30 MPa had stable properties in comparison with those grown at 0.1 MPa various hydrostatic pressures and temperatures.
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