Identification of a wheat allergen,Tri a Bd 36K,as a peroxidase |
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Authors: | Yamashita Hiromi Nanba Yoko Onishi Miki Kimoto Masumi Hiemori Miki Tsuji Hideaki |
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Institution: | Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja-shi, Okayama 719-1197, Japan. yamashit@fhw.oka-pu.ac.jp |
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Abstract: | A 36-kDa allergen, Tri a Bd 36K, was purified from wheat albumin and characterized. The protein was similar to barley peroxidase BP-1 both in its amino acid sequence and peroxidase activity. The enzyme seemed to contain L-fucose and D-mannose and the glycan moiety reacted with IgE antibodies in a patient's serum. |
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