贵州四个民族人群线粒体DNA Region Ⅴ的遗传多态性

目的研究世居贵州的侗族、仡佬族、土家族和彝族人群线粒体DNA RegionⅤ的遗传多态性。方法采用PCR-PAGE和克隆测序法对4个群体108份样本的mtDNA RegionⅤ进行序列分析。结果只检测到标准型和短型(即9-bp缺失)两种多态。贵州四个民族人群的平均9-bp缺失频率为22.2%,在侗族、仡佬族、土家族和彝族人群中依次为32.1%、22.6%、17.2%和15.0%。结论贵州四个民族mtDNA 9-bp缺失频率均较高,这与其地域分布相一致;贵州彝族和土家族显示了相似的缺失频率,提示两者可能有共同的祖先。

Polymorphism of mitochondrial DNA region in four ethnic populations from Guizhou

To study the polymorphism of mitochondrial DNA(mtDNA) region in Dong、Gelao、Tujia and Yi ethnic population from Guizhou. Method The region sequences in total of 108 samples from four ethnic populations were analyzed by polymerase chain reaction and polyacrylamide gel electrophoresis ( PCRPAGE) and clonal sequencing. Result Only two kinds of polymorphism were found in these populations. One was standard patter, the other was short patter( namely9bp deletion). The average frequency of 9bp deletion in four ethnic populations from Guizhou was 22.2%, and the frequencies of 9bp deletion in Dong、Gelao、Tujia and Yi population were 32.1%、 22.6%、17.2% and 15.0% respectively. Conclusion The frequencies of 9bp deletion are higher in four ethnic populations from Guizhou, which is consistent with their regional distribution, the frequencies of deletion in Tujia and Yi population are similar, this suggests they might originate from a common ancestry.