In vitro morphogenesis from excised leaf explants of Digitalis obscura L. |
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| Authors: | P. Pérez-Bermúdez M. C. Brisa M. J. Cornejo J. Segura |
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| Affiliation: | (1) Department of Plant Physiology, College of Pharmacy, University of Valencia, Avda. Blasco Ibañez 13, Valencia 10, Spain |
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| Abstract: | The morphogenic capacity of Digitalis obscura leaf explants cultured in vitro has been studied, noting factors promoting the differentiation of roots, buds and shoots as well as those promoting callus proliferation. Complete plant regeneration was obtained only by first culturing the leaf explants in a medium with NAA and BA to induce formation of buds, and subsequently transferring them to a medium without growth regulators to achieve the further development of shoots.Abbreviations BA benzyladenine - 2,4-D 2,4-dichlorophenoxyacetic acid - IAA indoleacetic acid - NAA naphthaleneacetic acid |
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