Enzymatic synthesis of perlauric acid using Novozym 435 |
| |
| Institution: | 1. School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China;2. College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China;3. Wilmar (Shanghai) Biotechnology Research and Development Center Ltd., Pudong New District, Shanghai 200137, China;1. Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC, Madrid, Spain;2. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, CEP 60455-760 Fortaleza, CE, Brazil;3. Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia |
| |
| Abstract: | For the biocatalytic synthesis of perlauric acid, Novozym 435 (Candida antarctica lipase immobilized on polyacrylic resin) was found to be the most active catalyst and toluene was the most suitable solvent. The effects of various parameters on conversion and rates of reaction were studied in the absence of mass transfer limitations. Reusability studies indicated that there was enzyme deactivation and from a preliminary study of the deactivation, it was observed that the deactivation obeys a pseudo-first-order model. Lineweaver–Burk plots indicated the formation of a ternary complex. A model based on ordered bi–bi mechanism was found to fit the initial rate data very well and using this model, some kinetic parameters were calculated by non-linear regression analysis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
