A Pathogenic Bacterium,Enterococcus faecalis,to the Beet Armyworm,Spodoptera exigua |
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| Institution: | 1. School of Bioresource Sciences, Andong National University, Andong, Korea;2. Department of Sericulture and Entomology Resources, Sangju National University, Sangju, Korea;1. Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India;2. Department of Chemistry, Durham University, DH1 3LE, United Kingdom;3. Department of science and Humanities, SRIT, Coimbatore 641010, Tamil Nadu, India;1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, China;2. Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212100, China;1. Department of Entomology, Universidade Federal de Viçosa, Minas Gerais, Brazil;2. Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), Viçosa, Minas Gerais, Brazil;3. Empresa Brasileira de Pesquisa Agropecuária – Embrapa Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brazil;4. Department of Entomology, University of Kentucky, Lexington, KY 40546, USA |
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| Abstract: | A bacterial disease was found in the beet armyworm, Spodoptera exigua (Hübner). Blackened body of the infected larvae was a typical symptom of the epizootic disease especially at the intersegmental areas. We isolated the bacteria from the hemolymph of the infected 5th instar larvae and identified the isolate as a gram-positive bacterium, Enterococcus faecalis. When the 4th instar larvae were injected with the bacteria, half lethal dose of the bacteria was estimated as 22,593 colony-forming units (cfu) per larva and half lethal time of the bacteria was estimated as 2 days at 107 cfu injection and 6 days at 108 cfu injection. The bacteria were strongly resistant to each 1,000 ppm of ampicillin, kanamycin, and streptomycin. They were, however, relatively susceptible to mixture (1,000 ppm) of different combinations of the three antibiotics. |
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