Automation of phage display for high-throughput antibody development |
| |
| Affiliation: | 1. Department of Medical Oncology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China;2. Biomedical Research Center and Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou, China;3. Department of Surgical Oncology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China;4. Department of Breast Surgery, The First affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China;5. Department of Colorectal Surgery, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China;6. Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, China |
| |
| Abstract: | Antibodies are important tools for the detection of diagnostic markers and are the most well-known examples of specific molecular interactions. We have developed automated technology that enables the selection of antibodies and other interacting molecules from large recombinant libraries. The physical link between phenotype and genotype in phage display allows selective isolation and amplification of a particular phage encoding a desired antibody fragment. Successive rounds of phage selection, amplification and screening are performed at high throughput, using a pin-based magnetic particle processor. The integration of this with existing DNA and protein array technology enables industrial screening of complex libraries and opens up a new level of functional genomic analysis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
