Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan |
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| Authors: | Anada Takahisa Matsunaga Hideshi Karinaga Ryouji Koumoto Kazuya Mizu Masami Nakano Koji Shinkai Seiji Sakurai Kazuo |
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| Institution: | Department of Chemical Process and Environments, The University of Kitakyushu, 1-1 Hibikino, Wakamatu-ku, Kitakyushu, Fukuoka 808-0135, Japan. |
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| Abstract: | A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. |
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