The secretome of Pleurotus sapidus |
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Authors: | Zorn Holger Peters Thilo Nimtz Manfred Berger Ralf G |
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Affiliation: | Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universit?t Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany. zorn@lci.uni-hannover.de |
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Abstract: | Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool. |
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Keywords: | 2‐D electrophoresis Basidiomycete Extracellular enzymes Fungi |
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