Epidermal growth factor stimulates DNA-synthesis of astrocytes in primary cerebellar cultures |
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Authors: | A Leutz Dr M Schachner |
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Institution: | (1) Institut für Neurobiologie, Universität Heidelberg, Heidelberg, Bundesrepublik Deutschland;(2) Institut für Neurobiologie, Universität Heidelberg, Im Neuenheimer Feld 504, 6900 Heidelberg, Federal Republic of Germany |
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Abstract: | Summary The capability of epidermal growth factor (EGF) to stimulate DNA synthesis in neural cells was investigated in primary cultures of early postnatal mouse cerebellum. At concentrations of 10-8M, EGF stimulates DNA synthesis in astrocytes, which were identified immunocytologically by the cell type-specific marker, glial fibrillary acidic (GFA) protein. Astrocytes express cell-surface receptors for EGF as can be shown by binding of 125I]-labeled EGF to live monolayer cultures. In the presence of 10% horse serum, EGF stimulates DNA-synthesis by a factor of about two-fold. Stimulation by EGF over control values is approximately 4-fold in the presence of 1% serum and 6 to 10-fold in the absence of serum. Absolute numbers of astrocytes are increased after more prolonged action of EGF. DNA-synthesis in neurons or oligodendroglia is not significantly stimulated by EGF. EGF enhances cell survival of serum-deprived cerebellar cultures. Fibroblast growth factor does not increase DNA-synthesis in astrocytes under the conditions used in this study.Abbreviations
BME
basal medium, Eagle's
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BME-BSA
basal medium, Eagle's containing 0.1% bovine serum albumin
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EDTA
ethylene-diamino-N, N-tetraacetic acid
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EGF
epidermal growth factor
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FGF
fibroblast growth factor
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GFA
glial fibrillary acidic
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HS
horse serum
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H] TdR
tritium-labeled thymidine
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PAP
peroxidase-anti-peroxidase
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PBS
phosphate-buffered saline
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SDS
sodium dodecyl sulfate
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TCA
trichloroacetic acid |
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Keywords: | Epidermal growth factor Astrocytes DNA-Synthesis Cerebellum Tissue culture (mouse) |
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