Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress |
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Authors: | Rhee Jee Eun Rhee Joon Haeng Ryu Phil Youl Choi Sang Ho |
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Affiliation: | Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea. |
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Abstract: | By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH. |
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Keywords: | Acid tolerance Vibrio vulnificus cadBA |
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