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| 硝酸羟胺对大鼠脑中Synaptobrevin和Syntaxin 的表达的影响 | |
| 引用本文： | 高亚兵,彭瑞云,王水明,王德文,陈建魁,马俊杰,张志毅.硝酸羟胺对大鼠脑中Synaptobrevin和Syntaxin 的表达的影响[J].中国组织化学与细胞化学杂志,2007,16(3):274-277. |
| 作者姓名： | 高亚兵 彭瑞云 王水明 王德文 陈建魁 马俊杰 张志毅 |
| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京,100850 2. 解放军307医院,北京,100850 3. 军事医学科学院仪器测试中心,北京,100850 |
| 摘 要： | 目的观察硝酸羟胺(HAN)对大鼠脑组织Synaptobrevin 2、Syntaxin的影响,探讨二者在HAN致脑损伤中的作用。方法应用免疫荧光双标记技术标记Synaptobrevin2和Syntaxin,用Hochest衬染细胞核,Radiance2100型激光共聚焦显微镜观察。结果Synaptobrevin 2及Syntaxin广泛分布在正常大脑皮质、海马及小脑,HAN注射后7d内,Synaptobrevin 2表达减弱,Syntaxin表达增强。结论Synaptobrevin 2和Syntaxin比例失调和相互作用减弱可能在HAN致脑损伤中发挥重要作用。 |
| 关 键 词： | 免疫荧光技术 硝酸羟胺 激光共聚焦扫描显微镜 |
| 修稿时间： | 2006年9月4日 |
THE EXPRESSION CHANGES OF SYNAPTOBREVIN AND SYNTAXIN IN RAT BRAIN TISSUES CAUSED BY INJECTION OF HYDROXYLAMMONIUM NITRATE |
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| Gao Yabing,Peng Ruiyun,Wang Shuiming,Wang Dewen,Chen Jiankui,Ma Junjie,Zhang Zhiyi.THE EXPRESSION CHANGES OF SYNAPTOBREVIN AND SYNTAXIN IN RAT BRAIN TISSUES CAUSED BY INJECTION OF HYDROXYLAMMONIUM NITRATE[J].Chinese Journal of Histochemistry and Cytochemistry,2007,16(3):274-277. | |
| Authors: | Gao Yabing Peng Ruiyun Wang Shuiming Wang Dewen Chen Jiankui Ma Junjie Zhang Zhiyi |
| Institution: | 1.Institute of Radiation Medicine, Academy of Military Medical Sciences, 1307 hospital of PLA , 2.National Center of Biomedical Analysis, Academy of Military Medical Sciences, Beijing 100850, China |
| Abstract: | Objective To study the expressions of Synaptobrevin 2 and Syntaxin in rat brain tissues and the damage mechanisms after the injection of Hydroxylammonium nitrate(HAN).Methods The antibodies to Synaptobrevin 2 and Syntaxin were labeled with red and green fluorescence dyes respectively.The cell nuclei were stanined with Hochest.The results were observed with Radiance2100 laser scanning confocal microscopy.Results Synaptobrevin 2 and Syntaxin were widely distributed in the normal cerebral cortex,hippocampus and cerebellum.The expression of Synaptobrevin 2 was up-regulated and that of Syntaxin was down-regulated in the brain 7d after HAN injection.Conclusion The expression disorder of Synaptobrevin 2 and Syntaxin may play an important role in the damages of brain tissue by the administration of hydroxylammonium nitrate. |
| Keywords: | Synaptobrevin2 Syntaxin |
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