L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches |
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Authors: | van der Kaaij Hengameh Desiere Frank Mollet Beat Germond Jacques-Edouard |
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Institution: | Nestlé Research Centre, Nestec, Ltd., CH-1000 Lausanne 26, Switzerland. |
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Abstract: | Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase. |
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