Liposome biodistribution by time resolved fluorimetry of lipophilic europium complexes |
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Authors: | Nathalie Mignet Quentin le Masne de Chermont Tatiana Randrianarivelo Johanne Seguin Cyrille Richard Michel Bessodes Daniel Scherman |
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Institution: | (1) CNRS-UMR8151; INSERM U640, Laboratoire Pharmacol. Chim. Genet., Université René Descartes Paris, 4, avenue de l’Observatoire, 75270 Paris cedex 06, France |
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Abstract: | The use of conventional fluorophores suffers from some limitations in biological fluids due to low signal/background ratio.
Today, this sensitivity issue might be reasonably improved thanks to lanthanide chelates, by selective detection of long decay
fluorescence. Use of pulsed light source time-resolved fluorimetry takes into account the fluorescence decay time of the lanthanide
chelates to gain sensitivity in biological media. Lipid-DTPA: Eu compounds have been prepared and incorporated into liposomes
to evaluate europium based detection of liposomes in biological media. Fluorescence emission was not modified by this incorporation.
Europium labelled liposomes were used for biodistribution studies and showed their use in this context. |
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Keywords: | Lanthanides Liposomes Pharmacokinetics |
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