Nitrate reductase and the membrane composition of pleiotropic chlorate resistant mutants of Escherichia coli K-12. |
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| Authors: | H C Grassi E Andrade J Puig |
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| Affiliation: | 1. The Department of Medical, University of Uppsala, Uppsala, Sweden;2. The Department of Physiological Chemistry, University of Uppsala, Uppsala, Sweden;3. Swedish University of Agricultural Sciences, The Biomedical Center, Uppsala, Sweden |
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| Abstract: | Attachment of rat hepatocytes to collagen but not to fibronectin substrata was efficiently inhibited by antibodies against the hepatocyte surface. Further analyses of this inhibition suggested that hepatocyte attachment to collagen involves cell surface antigens which are not identical to membrane bound fibronectin or collagen.Rabbit antibodies against rat fibronectin inhibited hepatocyte attachment to rat fibronectin but not to collagen or rabbit fibronectin. After plasmin digestion of fibronectin, peptides were isolated that lacked affinity for collagen but could serve as a substratum for hepatocyte attachment. These results suggested that attachment to fibronectin does not involve membrane bound fibronectin or collagen. |
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| Keywords: | To whom reprint requests must be sent. |
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