Dimerization of the P-glycoprotein in membranes |
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| Authors: | D Boscoboinik M T Debanne A R Stafford C Y Jung R S Gupta R M Epand |
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| Institution: | Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada. |
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| Abstract: | Plasma membranes from a CHO cell line, CHRC5, which exhibits multidrug resistance was studied using radiation inactivation analysis. The P-glycoprotein content of the membrane was determined by Western blots. Irradiation resulted in the loss of P-glycoprotein. The dependence of this loss on radiation dose corresponded to a target size of 250 kDa which is the molecular mass of a dimer of the P-glycoprotein. This is strong evidence to indicate that the P-glycoprotein self associates in the membrane. |
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