Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte <Emphasis Type="Italic">Prosopis strombulifera</Emphasis> |
| |
Authors: | Verónica Sgroy Fabricio Cassán Oscar Masciarelli María Florencia Del Papa Antonio Lagares Virginia Luna |
| |
Institution: | 1.Laboratorio de Fisiología Vegetal. Facultad de Ciencias Exactas,Universidad Nacional de Río Cuarto,Río Cuarto,Argentina;2.IBBM Facultad de Cs Exactas,Universidad Nacional de La Plata,La Plata,Argentina;3.Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas,Físico-Químicas y Naturales Universidad Nacional de Río Cuarto,Río Cuarto,Argentina |
| |
Abstract: | This study was designed to isolate and characterize endophytic bacteria from halophyte Prosopis strombulifera grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion or stress
homeostasis regulation. Isolates obtained from P. strombulifera were compared genotypically by BOX-polymerase chain reaction, grouped according to similarity, and identified by amplification
and partial sequences of 16S DNAr. Isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen
fixation, phosphate solubilization, siderophores, and phytohormones, such as indole-3-acetic acid, zeatin, gibberellic acid
and abscisic acid production, as well as antifungal, protease, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity.
A total of 29 endophytic strains were grouped into seven according to similarity. All bacteria were able to grow and to produce
some phytohormone in chemically defined medium with or without addition of a nitrogen source. Only one was able to produce
siderophores, and none of them solubilized phosphate. ACC deaminase activity was positive for six strains. Antifungal and
protease activity were confirmed for two of them. In this work, we discuss the possible implications of these bacterial mechanisms
on the plant growth promotion or homeostasis regulation in natural conditions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|