Affiliation: | 1 Institute of Genetics, Biological Research Center, P.O. Box 521, H-6701, Szeged, Hungary 2 Institute of Toxicology, Swiss Federal Institute of Technology and University of Zürich, schorennstrasse 16, CH-8603, Schwerzenbach, Switzerland |
Abstract: | Two genotypes were constructed to determine whether some of the mosaic spots, on which the SMART (somatic mutation and recombination test) procedures are based may arise through malsegregation of the chromosomes. Both arms of the metacentric third chromosomes were labelled with marker mutations, and in this way one- and two-arm events (the former representing rearrangements or point mutations, the latter representing malsegregation) could be recorded separately. Although several hundred clones were identified following exposure of larvae to X-rays, colchicine or vinchicine or vincristine (all known inducers of malsegregation), none arose as a consequence of two-arm events. This suggests that malsegregation of the chromosomes plays little, if any role in the formation of mosaic spots. Instead, the clones develop due to mitotic recombinations, deletions or point mutations. |