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Synthesis of (E)-9-(1-pyrenyl)-4-hydroxynon-2-enal, a fluorescent probe of the (E)-4-hydroxynon-2-enal with retained biological properties |
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| Authors: | Allevi P Anastasia M Cajone F |
| Institution: | a Dipartimento di Chimica e Biochimica Medica, Università degli Studi di Milano, Via Saldini 50, 20133 Milano, Italy b Istituto di Patologia Generale, Università degli Studi di Milano, Centro di Studio sulla Patologia Cellulare del CNR, via Mangiagalli 31, 20133 Milano, Italy |
| Abstract: | (E)-9-(1-pyrenyl)-4-hydroxynon-2-enal (FHNE), a fluorescent probe of (E)-4-hydroxynon-2-enal (HNE) is synthesised in seven steps and in 35% overall yield, starting from commercially available 1-pyrencarboxyaldehyde. When incubated with cultured HeLa cells this fluorescent probe penetrates cells and particularly concentrates in the region surrounding the nucleus. As the parent compound, HNE it is able to induce the activation of heat shock factor (HSF) and it is able to induce the binding of HSF to heat shock element (HSE). |
| Keywords: | Lipid peroxidation (E)-4-hydroxynon-2-enal (E)-9-(1-pyrenyl)-4-hydroxynon-2-enal Heat shock proteins |
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