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| 茶树叶绿体及其蛋白的分离研究 | |
| 引用本文： | 张立明,刘亚军,王云生,高丽萍,夏涛. 茶树叶绿体及其蛋白的分离研究[J]. 激光生物学报, 2011, 20(6): 802-808. DOI: 10.3969/j.issn.1007-7146.2011.06.018 |
| 作者姓名： | 张立明 刘亚军 王云生 高丽萍 夏涛 |
| 作者单位： | 1. 安徽农业大学生命科学学院,安徽合肥,230036 2. 农业部茶叶生物化学与生物技术重点实验室,安徽合肥,230036 3. 安徽农业大学生命科学学院,安徽合肥230036;农业部茶叶生物化学与生物技术重点实验室,安徽合肥230036 |
| 基金项目： | 国家自然科学基金项目项目，高校博士点基金项目，安徽省自然科学基金项目 |
| 摘 要： | 茶树叶片叶绿体的有效分离纯化是进行茶树叶绿体代谢组学和蛋白质组学研究的基础.本文以茶树鲜叶为材料,通过叶绿体得率、希尔反应等纯度和完整度指标,比较了Percoll密度梯度离心法和蔗糖密度梯度离心法对叶绿体分离纯化的效果；通过蛋白质含量和SDS-PAGE电泳图谱,比较了涨破法和冻融法对叶绿体蛋白的提取效果.结果发现Per... |
| 关 键 词： | 茶树 叶绿体 儿茶素 合成酶 |
A Study on Isolation of Chloroplasts and Relative Protein from Tea Leaves |
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| ZHANG Liming,LIU Yajun,WANG Yunsheng,GAO Liping,XIA Tao. A Study on Isolation of Chloroplasts and Relative Protein from Tea Leaves[J]. ACTA Laser Biology Sinica, 2011, 20(6): 802-808. DOI: 10.3969/j.issn.1007-7146.2011.06.018 | |
| Authors: | ZHANG Liming LIU Yajun WANG Yunsheng GAO Liping XIA Tao |
| Affiliation: | ZHANG Liming,LIU Yajun,WANG Yunsheng(1,2),GAO Liping(1＊)XIA Tao(2＊) (1.College of Biology Science,Anhui Agricultural University,Hefei 230036,Anhui,China;2.Key Lab of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036,Anhui,China) |
| Abstract: | The effective isolation and purification of chloroplasts is a basic for tea chloroplasts metabonomics and proteomics research.In this paper,using fresh tea leaves as materials,the effect of isolation and purification of chloroplasts were comparably analyzed between Percoll density gradient centrifugation and sucrose density gradient centrifugation through comparing the chloroplast yield,Hill reaction.The effect of chloroplast protein extracting were compared between freezing-thawing method and distension method through comparing the extraction yield of protein and the SDS-PAGE pattern. The result showed that Percoll density gradient centrifugation and freezing-thawing method were suitable for isolating intact chloroplasts and chloroplast protein extraction.On this basis,the fresh tea leaves and tea chloroplasts proteins were preliminary comparably analyzed by two-dimensional electrophoresis methods and the enzyme of tea catechins synthesis related,PAL,DFR/LAR and ANR were detected in tea chloroplasts. |
| Keywords: | tea plant chloroplast catechins synthetase |
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