In vitro rooting of almond (Prunus dulcis Mill.) |
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Authors: | Phillip J Ainsley Graham G Collins Margaret Sedgley |
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Institution: | (1) Department of Horticulture Viticulture and Oenology, Plant Research Centre, Adelaide University, Waite Campus, PMB1, 5064 GLEN OSMOND, South Australia, Australia |
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Abstract: | Summary Shoot cultures of the paper shell almond (Prunus dulcis Mill.) cultivars ‘Ne Plus Ultra’ and ‘Nonpareil’ were subcultured for 4 wk at 4°C on growth regulator-free basal medium under
low light conditions. Elongated shoots were excised and their response to a range of rooting treatments determined. Various
concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid were compared over a range of incubation periods
to determine the optimum auxin for root formation. In addition, the effect of shoot base shading, phloroglucinol (PG), and
basal salt composition were examined. The treatment resulting in the best rooting of both cultivars was shoot insertion for
12 h into water-agar (0.6% w/v) with 1.0 mM IBA, followed by 2 wk in basal medium without auxin but with 100.0 μM PG. Explants were maintained under dark conditions for 3 d at the start of the treatment period, then exposed to light. Extending
the darkening period did not improve rooting ability. Whilst half-strength Murashige and Skoog basal medium was suitable for
rooting “Ne Plus Ultra’ shoots, full-strength Almehdi and Parfitt medium resulted in the best rooting of ‘Nonpareil’. Under
these conditions, 60.0% of explants developed adventitious roots. |
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Keywords: | adventitious roots indole-3-butyric acid α -naphthaleneacetic acid phloroglucinol Prunus dulcis rooting |
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