| 核多角体病毒感染昆虫细胞的DNA拓扑异构酶性质的研究 |
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| 引用本文： | 李永方,王平,巫爱珍. 核多角体病毒感染昆虫细胞的DNA拓扑异构酶性质的研究[J]. 病毒学报, 1988, 0(4) |
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| 作者姓名： | 李永方 王平 巫爱珍 |
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| 作者单位： | 中国科学院上海生物工程中心(李永方，王平)，中国科学院上海生物工程中心(巫爱珍) |
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| 摘 要： | 核多角体病毒(Nuclear polyhedrosis virus)的核酸是双股环状DNA,在病毒颗粒中呈超螺旋状态。超螺旋DNA复制时,一般皆有超螺旋解旋过程。为了解这一机制,本文报道了从NPV感染的家蚕中肠组织中分离细胞核,经过羟基磷灰石、磷酸纤维素柱层析,ssDNA-纤维素亲和层析,纯化了DNA拓扑异构酶I,SDS-PAGE测定分子量为47kd,最适Mg~(++)浓度约为5mM。AcNPV感染的TN368细胞DNA拓扑异构酶I总活力较正常细胞酶活力高1～3倍,且活力的提高与病毒增殖平行。讨论了昆虫细胞DNA拓扑异构酶I的性质及其与NPV复制的关系。
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| 关 键 词： | 核多角体病毒 超螺旋DNA 松弛型DNA 拓扑异构酶 |
INSECT DNA TOPOISOMERASE AND THE REPLICATION OF NPV |
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| Li Yongfang Wang Ping Wu Aizhen. INSECT DNA TOPOISOMERASE AND THE REPLICATION OF NPV[J]. Chinese journal of virology, 1988, 0(4) |
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| Authors: | Li Yongfang Wang Ping Wu Aizhen |
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| Affiliation: | Shanghai Center of Biotechnology of Chinese Academy of Sciences |
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| Abstract: | Nuclear polyhedrosis virus ( NPV ) is one of the baculovirus which consists of supercoiled DNA in the virion. This paper describes the DNA topoisomerase I isolated from the mid-gut of silkworm , Bombyx mori and TN368 celis infected with NPV. The topoisomerase I was purified by hydroxyapatite chromatography, phosphorocellulose chromatography and DNA affinity chromatography. The molecular weight of DNA topoisomerase was 47K dalton. Mg++ was not essential but made the enzymatic activity of the DNA topoisomerase increased greatly. The activity of the DNA topoisomerase from infected TN368 celis increased parallelly with the multipli-cation of NPV. It was suggested that the DNA topoisomerase should bei-nvolved in the replication of NPV. |
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| Keywords: | Nuclear polyhedrosis virus Supercoiled DNA Relaxed DNA Topoisomerase |
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