Improved translational efficiency of subtilisin YaB gene with different initiation codons in Bacillus subtilis and alkalophilic Bacillus YaB |
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| Authors: | CM Yeh HK Chang HM Hsieh K Yoda M Yamasaki & YC Tsai |
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| Institution: | Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan,;Department of Food Science, National Chung-Hsing University, Taichung, Taiwan,China,;Department of Biotechnology, The University of Tokyo, Tokyo, Japan |
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| Abstract: | The ale gene specifying the subtilisin YaB produced by alkalophilic Bacillus YaB, has an unusual start codon UUG. Changing this codon to AUG and GUG increasedexpression of the ale gene in B. subtilis DB104 and in an ale deficient mutant strain YaB-DEC4. The relative translational efficiency order of the threeinitiation codons is AU G > GU G > UUG in B. subtilis DB104 and in YaB-DEC4. These data suggest that the preferred initiation codon is AUG for ale gene expression in Bacillus . |
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