Lutein-chlorophyll-a energy transfer in detergent micelles |
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Authors: | J Szabad E Lehoczki L Szalay K Csatorday |
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Institution: | 1. Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary 2. Institute of Biophysics, József Attila University, Szeged, Hungary
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Abstract: | Absorption, fluorescence and fluorescence excitation spectra were determined for equimolar mixed micellar detergent solutions of lutein and chlorophyll-a in the concentration range from 9·10?6 to 1.8·10?4 M, with detergent (triton-X100) concentrations from 3·-10?4 to 7·10?3 M. In the range of detergent concentrations studied the pigments incorporated into the detergent micelles attained a high local concentration (0.1 to 0.01 M), reminiscent of pigment concentration within the chloroplast. A lutein → chlorophyll-a energy transfer with an efficiency of about 15% was found in these systems. In dilute (9·10?6 M) pigment solution with concentrated (7·10?3 M) detergent practically no transfer is observed. The extent of aggregation and the efficiency of transfer depend on the composition of the system. The aggregation of chlorophyll-a is partly inhibited by lutein molecules. It is shown that the energy transfer efficiency as function of distance follows anr ?3 relationship,R 0 being 22 å. |
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