Binding of adriamycin to sulphated mucopolysaccharides |
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Authors: | M Menozzi F Arcamone |
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Institution: | Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 101, Japan |
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Abstract: | Two distinct, Mn2+- and Mg2+-dependent enzymes with ribonuclease H (RNase H) activities, which degrade RNA-DNA hybrid have been purified from rat liver nuclei. These two enzymes were eluted at 0.16 M and 0.37 M of potassium chloride in phosphocellulose chromatography, respectively, and further purified by blue Sepharose. They are distinguished from one another by their ionic requirement, molecular weight, sedimentation coefficient, optimal pH, sensitivity to the -SH reagent and mode of cleavage. |
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