Activation of 2,4-dinitrophenol-stimulated ATPase activity in cauliflower and corn mitochondria. |
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Authors: | D W Jung J B Hanson |
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Affiliation: | Department of Botany, University of Illinois, Urbana, Illinois 61801 USA |
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Abstract: | Cauliflower mitochondria do not have a 2,4-dinitrophenol-stimulated ATPase unless they are permitted a brief period of respiration (respiratory priming) or are preincubated for an extensive period with ATP (self-priming). Both priming processes are dependent on Mg2+, and are collapsed by 2,4-dinitrophenol in the absence of ATP. Corn mitochondria, which have an endogenous DNP-ATPase, contain significantly more Mg2+ and adenine nucleotides than cauliflower mitochondria. Primed cauliflower mitochondria have Mg2+ content comparable to corn mitochondria. Cauliflower mitochondria will actively accumulate adenine nucleotides through atractyloside-insensitive sites. It appears that priming consists of creating an electrochemical potential which is needed for accumulation of Mg2+ or adenine nucleotides or for charge compensation of the exchange. |
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