Suppression of mouse lymphocyte responses to mitogens in vitro by Trypanosoma cruzi |
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Authors: | J R Maleckar F Kierszenbaum |
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Institution: | Department of Microbiology and Public Health, Michigan State University, East Lansing, MI 48824-1101, U.S.A. |
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Abstract: | Maleckar J. R. and Kierszenbaum F. 1984. Suppression of mouse lymphocyte responses to mitogens in vitro by Trypanosoma cruzi. International Journal for Parasitology14: 45–52. The ability of T. cruzi to inhibit mitogen-induced mouse lymphocyte responses was studied to find out if the organism itself is involved in the production of the immunosuppression that occurs during the acute phase of Chagas' disease. Significant suppression of normal spleen cell responses to concanavalin A (a T cell-specific mitogen) or to bacterial lipopolysaccharide (a B cell-specific mitogen) were seen when the concentration of either trypomastigote or epimastigote forms of the parasite reached or exceeded 2.5 × 106 organisms/ml in the cultures. The inhibitory effect was noted over wide ranges of concentrations of either mitogen. Since spleen cells stimulated with mitogenic solutions that had been absorbed with 1 × 107 parasites/ml produced significant responses, the suppressive effect could not be attributed just to mitogen removal by the parasites. Preparations of T. cruzi disrupted by freezing and thawing also inhibited mitogen-induced responses. This indicated that production of suppression was not a result of parasite competition for essential medium nutrients and that trypanosome viability was not required. Suppression was demonstrable only when the parasites were incorporated into the cultures within 12 h after mitogenic stimulation. These results taken together indicate that T. cruzi has the ability to modulate directly or indirectly lymphocyte function by interfering with the initial stages of commitment to lymphoproliferation. |
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Keywords: | Chagas' disease epimastigote trypomastigote immunosuppression lymphoproliferation Con A concanavalin A FTE epi-mastigotes subjected to five cycles of freezing and thawing FTT trypomastigotes subjected to five cycles of freezing and thawing LPS bacterial lipopolysaccharide |
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