Soluble E-Selectin Enhances Intercellular Adhesion Molecule-1 (ICAM-1) Expression in Human Tumor Cell Lines |
| |
Authors: | Bianca M. Wittig,Ulrich Treichel,Roman Blaheta,Thomas Schreiter,Andreas Schwarting,Karl-Hermann Meyer zum Bü schenfelde,Werner Mayet |
| |
Affiliation: | aFirst Department of Internal Medicine, Johannes Gutenberg-University of Mainz, Langenbeckstrasse 1, 55101, Mainz;bGeneral Department of Surgery, University of Frankfurt, Theodor Stern-Kai 7, 60590, Frankfurt, Germany |
| |
Abstract: | E-selectin mediates neovascularization via its soluble form, while its membrane-bound form initiates binding of tumor cells to vascular endothelium. Therefore, it was studied whether soluble E-selectin regulates further adhesion molecules on tumor cells. In tumor cells but not in related nonmalignant cells, intercellular adhesion molecule (ICAM)-1 expression was strikingly increased from 5 to 68% positive cells byin vitroinoculation of a recombinant E-selectin–IgG1 within 24 h, as analyzed by flow cytometry. The absence of changes in the expression of vascular cell adhesion molecule, integrin ligands (CD11a, CD18, integrin α4), and sialyl-Lewis X indicates a specific effect of soluble E-selectin on ICAM-1. A cell adhesion assay revealed that the enhanced adhesion of T-cells to tumor cells mediated by soluble E-selectin-induced ICAM-1 expression was at a maximum after a 12-h incubation period. Therefore, ICAM-1 regulation on tumor cells might be a mechanism of immune escape. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|