Aspergillus niger mutants with increased glucose oxidase production |
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Authors: | John Markwell Laura G Frakes Eugene C Brott John Osterman Fred W Wagner |
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Institution: | (1) Department of Biochemistry, University of Nebraska, 68583 Lincoln, Nebraska, USA;(2) School of Biological Sciences, University of Nebraska, 68583 Lincoln, Nebraska, USA |
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Abstract: | Summary
Aspergillus niger NRRL-3, an organism used for the industrial scale production of d-gluconic acid and glucose oxidase (EC 1.1.3.4), was subjected to mutagenesis and selection for acid production on diagnostic media containing methyl red. The plates contained 0.1 M d-glucose, a concentration that does not produce a color change in the medium surrounding mycelia of the parental strain under the conditions employed. Mutagenized spores yielded occasional colonies which were able to grow rapidly and were surrounded by a reddish zone. A number of such presumptive mutants were selected and isolated. Twenty-six such strains were grown in shaken cultures with liquid media containing 0.01, 0.1 or 0.5 M d-glucose, harvested, disrupted and the specific activity of d-glucose oxidase determined. Seven of the mutant strains had glucose oxidase specific activities markedly higher than the parental strain.Paper No. 8393, Nebraska Agricultural Research Division. |
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