Effect of iron on fluconazole activity against Candida albicans in presence of human serum or monocyte-derived macrophages |
| |
Authors: | Minn Yuriko Brummer Elmer Stevens David A |
| |
Institution: | (1) Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center, USA;(2) California Institute for Medical Research, San Jose, USA;(3) Division of Infectious Diseases and Geographic Medicine, Stanford University Medical School, Stanford, CA, USA |
| |
Abstract: | Human serum, transferrin, and apotransferrin are known to profoundly inhibit the growth of Candida albicans by iron deprivation.
On the other hand, iron overload (iron saturated transferrin) is a serious risk factor for candidiasis in newborn and in leukemic
patients. We tested the efficacy of fluconazole and the previously demonstrated synergy of fluconazole and effector cells
against C. albicans under iron overload conditions where efficacy might be diminished. We confirm that exogenous iron completely
reversed the inhibitory effect of human serum and report that the efficacy of fluconazole against C. albicans was not significantly
compromised in a 24 h assay system. Although exogenous iron inhibited fungistatic activity of monocyte-derived macrophages,
it did not interfere with the synergistic candidacidal activity of fluconazole and monocyte-derived macrophages. In 72 h assays,
where fluconazole had candidacidal activity, exogenous iron did not compromise efficacy of fluconazole, and fluconazole activity
was often increased. These in vitro results suggest that effectiveness of fluconazole therapy would not be compromised in
iron overload situations in vivo.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | Fluconazole Iron Candida albicans macrophage monocyte-derived |
本文献已被 SpringerLink 等数据库收录! |
|